| 1. | Inspection method on newcastle disease virus in poultry for export
出口家禽新城疫病毒检验方法 |
| 2. | The investigation on the molecular epizootiology of newcastle disease virus
中国部分地区新城疫病毒的分子流行病学研究 |
| 3. | Newcastle disease virus
新城疾病病毒 |
| 4. | Construction of baculovirus transfer vectors for expression of core antigens of newcastle disease virus
核心抗原重组杆状病毒转移载体的构建 |
| 5. | The causative agent of the disease , newcastle disease virus ( ndv ) , is a member of the paramyxoviridae whose genome is a non - segmented single - strand negative - sense rna
该病的病原新城疫病毒( newcastlediseasevirus , ndv )属副粘病毒科，为有囊膜的负链rna病毒。 |
| 6. | In this study five recombinant plasmids containing hn ( hemagglutinin - neuraminidase ) genes of ndv ( newcastle disease virus ) were constructed , hn genes were amplified by reverse transcriptase - polymerase chain reaction ( rt - pcr ) and were inserted directly into eukaryotic expression plasmid pcdna3
应用反转录-聚合酶链反应( rt - pcr )获得两株新城疫病毒( ndv )血凝素-神经氨酸酶( hn )基因cdna ，然后定向插入真核表达质粒pcdna3中，构建了含hn基因的重组质粒。 |
| 7. | Newcastle disease virus ( ndv ) is grouped under the family of paramyxoviridae , it causes serious infectious disease and incurred great losses to poultry industry . f and hn protein were main glycoproteins , which affect the virulence of the virus
新城疫病毒( newcastlediseasevirus ， ndv )属于副粘病毒科( paramyxoviridae )副粘病毒亚科( paramyxovirinae )腮腺炎病毒属( rubulavirus )的成员，其主要结构蛋白融合蛋白( f )和血凝素-神经氨酸酶( hn )与其致病性密切相关。 |
| 8. | Newcastle disease virus ( ndv ) strain 695 , a thermostable nature avirulent strain , were replicated in embryonated chicken eggsand its rna was extracted from allantoic fluid . referred to the reported sequence of f gene , a pair of primers were designed and synthesized . f gene of ndv b95 strain was amplified by rt - pcr , the pcr products were checked by agrose gel electrophoresis and purified by agrose gel fracion method
利用从国外引进的新城疫热稳定性天然弱毒b _ ( 95 )株接种spf鸡胚繁殖病毒，经处理后提取病毒的基因组rna ，参考国内外发表的ndv融合蛋白基因序列，设计一对特异性引物，经反转录聚合酶链式反应( rt - pcr )扩增出约1700bp大小的特异性片段，将此片段回收纯化后，利用t - a克隆技术将其克隆到pgem - t - easy克隆载体中，再转化大肠杆菌jm109感受态细胞，转化后经分子量比较、 pcr鉴定和酶切分析筛选阳性克隆。 |
| 9. | In recent years , the outbreak of nd appear some new characters , it is reported that the amount of immunity defeat on nd increased , even chicks with high hi titer can be infected by ndv virulent strain . the gene - engineering vaccine is badly needed to control nd outbreak . so newcastle disease virus strain b95 , a naturally avirulent and heat stable strain , was introduced from australia
新城疫是国际兽疫局公布的16种烈性传染病之一。近年来，新城疫的爆发出现了许多新特点，免疫失败报道增多，甚至抗体水平较高的鸡群也发病。为控制新城疫的爆发，急需研制基因工程苗。 |
| 10. | A reverse - transcriptase polymerase chain reaction ( rt - pcr ) based technique was developed to detect newcastle disease virus ( ndv ) of different poultry species origin . four oligonucleotide primers , based on the differences of nucleotide sequence at the cleavage site of fusion ( f ) protein gene between virulent and non - virulent strains of apmv - 1 , were designed to amplify specific dna fragment from different viruses
依据apmv - 1融合蛋白( f )基因裂解位点的核苷酸序列与其毒力的相关规律，分别设计合成了四条寡核苷酸引物，建立了一个可迅速检测不同禽源apmv - 1并可鉴定强、弱毒株的逆转录酶?聚合酶链式反应( rt - pcr )技术。 |
